rescue实验 - 哔哩哔哩
2023年12月17日 · A蛋白的过表达（overexpression, oe）用来抵消B蛋白的下调（通过siRNA敲降）所造成的影响。 通过比较不同实验组的细胞活性，可以观察到过表达A蛋白是否能够挽救B蛋白下调引起的细胞活性减少。
如何设计rescue实验 - 知乎 - 知乎专栏
2021年5月1日 · 如何设计rescue实验. rescue实验是机制研究的必备操作，如何设计，咱们根据一张模式图去理解。. 酸性微环境诱导激活转录因子FOXX1高表达激活下游靶分子MAZ高表达，从而促进自噬阻碍了EMT过程。.
Rescue挽救实验是什么？如何设计？ - 知乎专栏
2023年1月29日 · 单因素rescue是指围绕单一因素进行的实验，如以上例子中仅针对基因X进行实验，通过调控基因X观察癌症转移的变化。实际应用中，该因素可以是基因、信号通路、药物等变量。
Attenuated Protein Expression Vectors for Use in siRNA Rescue ...
2016年10月15日 · Transient transfection of small interfering RNA (siRNA) provides a powerful approach for studying cellular protein functions, particularly when the target protein can be re-expressed from an exogenous siRNA-resistant construct in order to rescue the knockdown phenotype, confirm siRNA target specificity and support mutational analyses.
High-throughput synthetic rescue for exhaustive characterization …
2020年4月8日 · The overexpression of protein 3 can overcome protein 1 mutation despite being of low constitutive activity at normal expression rate . For instance, the overexpression of BRL1 involved in changing the nuclear membrane composition can suppress the inhibited nuclear protein import caused by a mutation in NUP116 [ 39 ] (Fig. 4 b).
Nutrient-sensing alteration leads to age-associated distortion of ...
5 小时之前 · However, the defects related to a higher percentage of mature EEs and a lower percentage of mature ECs in aging individuals were only rescued by the overexpression of (1) Notch, (2) Escargot, and ...
Specific and efficient gene knockout and overexpression in …
2022年12月12日 · This enables specific deletion of target genes in brown adipocytes within 6 weeks by local injection of AAV-sgRNA into interscapular brown adipose tissue. We also describe an adiponectin-promoter-driven AAV vector to express sgRNA-resistant cDNA-encoded protein for subsequent rescue.
CMYC-initiated HNF1A-AS1 overexpression maintains the …
2024年4月23日 · The results of luciferase assay showed that overexpression of HNF1A-AS1 reversed the inhibitory effect of miR-150-5p on pmirGLO-β-catenin 3′ UTR luciferase activity in GC cells, whereas HNF1A ...
CTNND2 moderates the pace of synaptic maturation and links …
Outline. More. Highlights. •. CTNND2 regulates the pace of synaptic maturation and intrinsic excitability. •. CTNND2 is necessary for the synaptic accumulation of the ID/ASD protein SYNGAP1. •. SRGAP2A inhibition by human-specific SRGAP2C enhances CTNND2 level at …
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