TUNEL assay - Wikipedia
Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the 3′- hydroxyl termini in the double-strand DNA breaks generated during apoptosis.
TUNEL staining methods and kits - Abcam
TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) staining, also called the TUNEL assay, detects the DNA breaks formed when DNA fragmentation occurs in the last phase of apoptosis. Contents
TUNEL Assays | Thermo Fisher Scientific - US
TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assays are a widely used technique in the field of molecular biology and cell death research.
Detection of apoptosis by TUNEL assay - PubMed
Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis.
TUNEL Assay - an overview | ScienceDirect Topics
TUNEL Assay is a technique that allows for the detection of both single and double strand DNA breaks, using fluorescent or light microscopy, and can be coupled with flow cytometry for high-throughput analysis of thousands of samples, ensuring accuracy and reproducibility.
TUNEL assay – Encyclopedia of Biological Methods
TUNEL is used to detect cells with massive amount of DNA damage, especially apoptotic cells, as DNA fragmentation is one step of the apoptotic death program. To perform the TUNEL assay, samples are first fixed, then solubilized.
TUNEL Staining: An Overview - News-Medical.net
2019年2月25日 · Otherwise known as the TUNEL assay, TUNEL staining is used to detect DNA breaks formed during the final phase of apoptosis, when DNA fragmentation takes place. Principles of TUNEL Staining
Click-iT TUNEL Alexa Fluor Imaging Assay Protocol
The Click-iT TUNEL Alexa Fluor imaging assay contains all components needed to accurately and reliably detect apoptosis on adherent cells grown on coverslips or a 96-well microplate, and includes DNase I to generate strand breaks as a positive control.
TUNEL Apoptotic Cell Detection in Tissue Sections: Critical …
TUNEL, i.e., terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling, has become a widely used staining method to assist in detection of apoptotic cells in tissue sections.
Analysis of apoptosis by cytometry using TUNEL assay
Several variants of the methodology that is based on fluorochrome-labeling of 3′-OH termini of DNA strand breaks in situ with the use of exogenous terminal deoxynucleotidyl transferase (TdT), commonly defined as the TUNEL assay, have been developed by us. This Chapter describes the variant based on strand breaks labeling with Br-dUTP that is ...