请教一下关于同位素标记的糖 - 生物科学 - 小木虫 - 学术 科研 互动 …
[U-13C6]glucose表示C-13均匀标记在葡萄糖的6个碳上，6个碳上的标记概率均等。通常这种标记物是经生物合成途径得来的。
技术介绍 | 靶标代谢流分析 - 知乎 - 知乎专栏
BIOTREE能量代谢之靶标代谢流解决方案： 主要集中在能量代谢明星通路精细化代谢速率研究， 包含糖酵解途径、三羧酸循环、磷酸戊糖途径。 糖酵解途径 (EMP)是指糖原或葡萄糖分子分. 基于 ^ {13}C 或者 ^ {15}N 标记的靶标代谢流分析能够系统地定量细胞或者组织内特定代谢通路代谢网络的流量分布及各代谢途径的相对贡献，其优点是可以利用细胞内代谢物的质量同位体信息的分析方法，不但在很多情况下…
Uniformly-labeled [13C]glucose - National Cancer Institute
A non-radioactive, naturally occurring carbon 13 (13C) glucose isotopomer in which all six carbons are 13C, with potential imaging application. Upon administration of uniformly-labeled [U-13C] glucose, the glucose is taken up and metabolized by tumors and the 13C-containing metabolites can be imaged by 13C nuclear magnetic resonance (NMR ...
A guide to 13 C metabolic flux analysis for the cancer biologist
2018年4月16日 · Generally, 13 C-glucose tracers are best for determining fluxes in upper metabolism (e.g., glycolysis and PPP), while 13 C-glutamine tracers typically produce better resolution of fluxes in lower...
U-13C 本应用简报展示了使用 葡萄糖作为示踪物对人癌细胞系的定性代谢流分析。 在 6546 LC/Q-TOF 系统上采集数据,并利用 MassHunter VistaFlux 进行分析。 该研究HepG2 证明了丙酮酸羧化酶敲低对细胞的三羧酸循环中葡萄糖代谢流的影响。 前言丙酮酸羧化酶 (PC) 是一种线粒体回补代谢物提取稳定同位素标记可提供有关细胞内代谢的酶,其产生用于生物合成目的的柠檬酸循用 150 mmol/L 乙酸铵快速清洗后,用 独特信息。 尽管代谢组学分析提供了不同环中间物。 该酶对于肝 …
Metabolism of [U-13C]glucose in Human Brain Tumors In Vivo
Stable isotope tracers such as 13 C-enriched glucose are valuable tools to probe metabolism in vivo, because the distribution of 13 C in metabolites downstream of glucose, analyzed by 13 C nuclear magnetic resonance (NMR) spectroscopy, provides a wealth of information about metabolism in the tissue of interest. Because these tracers are not ...
In vivo [U-13C]glucose labeling to assess heart metabolism in …
This study aimed to implement an in vivo [U-13 C]glucose labeling strategy to assess glucose carbon metabolism in mice and use this methodology to investigate potential metabolic alterations in pressure-overload and volume-overload LV hypertrophy.
Estimating gluconeogenesis with [U-13C]glucose: molecular ... - PubMed
Both groups estimate gluconeogenesis from cycling of [U-13C]glucose to lactate and back to glucose, detected by mass spectrometry. Landau's approach is based on analysis of labeled molecules, whereas Tayek and Katz's is based on labeling of carbon atoms by use of the concept of "molar enrichment," which weights each mass isotopomer by the ...
Uniformly-labeled [U-13C]glucose - Semantic Scholar
A non-radioactive, naturally occurring carbon 13 (13C) glucose isotopomer in which all six carbons are 13C, with potential imaging application. Upon administration of uniformly-labeled [U-13C] glucose, the glucose is taken up and metabolized by tumors and the 13C-containing metabolites can be imaged by 13C nuclear magnetic resonance (NMR ...
uniformly-labeled [U-13C] glucose - Virginia Cancer Specialists
2024年10月22日 · A non-radioactive, naturally occurring carbon 13 (13C) glucose isotopomer in which all six carbons are 13C, with potential imaging application. Upon administration of uniformly-labeled [U-13C] glucose, the glucose is taken up and metabolized by tumors and the 13C-containing metabolites can be imaged by 13C nuclear magnetic resonance (NMR ...